This study was carried out to detect the elevated serum Anti- streptolysin O (ASO) in patients which were coming in OPD of Regional Research Institute of Unani Medicine with various clinical conditions during the period of January 2011 to December 2012. The serum samples were tested for ASO antibodies by latex agglutination test. Total 205 patients including 30.24 % male and 69.7 % female were tested for ASO serum levels. 60 were found positive and 145 were negative. In 60 positive cases 16 were male and 44 were female. The prevalence of ASO antibody in total cases was 29.26 %. The prevalence in male was 25.8 % and in female was 30.76 %. The presence of elevated streptococcal antibody titers in such a population probably reflects a medium background prevalence of streptococcal infections.