In view of probability of adulteration especially in unorganized drugs, this study was designed for standardization of Dammul Akhawain (Dragon’s blood) to generate data for future reference. The study was carried out on a samples of Dragon’s blood obtained from the plant Pterocarpus marsupium Roxb, considered as the standard sample. The study comprised of morphology, physicochemical study, physical constants, preliminary phytochemical study and spectrophotometery. Since in literature parameters for this have not been mentioned sufficiently, therefore, the findings of this study may be considered as standard for quality assessment of available sample of Dragon’s blood.