Kabideen (Syrup) is a Unani polyherbal formulation manufactured and marketed by a reputed Unani Pharmaceutical establishment Dawakhana Tibbiya College, Aligarh Muslim University Aligarh, India. This preparation is being prescribed by the physicians of traditional medicines in the management of liver disorders since last many years. Although the reports of physicians suggest that it is clinically very effective hepatoprotective drug, but scientific studies have not been conducted so far on this product to validate the claims of Unani physicians. Therefore the present study was designed to investigate the self prepared (SP) and market sample (MS) of Kabideen for hepatoprotective activity against paracetamol induced liver damage in albino rats of either sex at a dose of 5.25 ml/kg body weight. Various biochemical parameters of liver function including serum total bilirubin, total protein, aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total cholesterol and lipid peroxidation were measured to assess the effect of the test drug on paracetamol induced hepatic damage. The histopathological study of liver tissue was also conducted. Results of the study revealed elevated level of marker enzymes in the animals treated with paracetamol (750 mg/kg intraperitoneally), indicating severe hepatic damage, whereas significant reduction in the serum markers were seen in the animals treated with two samples of Kabideen indicating significant hepatoprotective effect possessed by the test drug. However, the effect produced by the self prepared sample was more striking.