With global realization that use of synthetic drugs is not safe on the long run, the medical fraternity at large is looking at alternatives from natural sources to combat diseases particularly those in which conventional modern system of medicine has little to offer. This realization on the one hand has increased demand for herbal drugs and on the other hand need for quality standardization of these drugs has gone up. Central Research Institute of Unani Medicine, Hyderabad being engaged in multidisciplinary research in Unani Medicine, working on standardization of herbal drugs used in this system of medicine. One such drug “Habb-e-Paan” which is prescribed in Unani system for Aatishak (Syphillis), Fasad-e-Dam (Putrefaction of Blood) has been taken up for standardization by modern techniques, so as to ascertain its quality. The parameters which were carried out are pharmacognostic studies, physico-chemical parameters, phytochemical screening, High performance thin layer chromatography, microbial load, aflatoxins, heavy metals, and pesticidal residues revealing specific identities for the particular drug and to evaluate pharmacopoeial standards. Results suggest that the drug is safe for therapeutic use and its batch to batch identification for quality control is possible on the basis of present study.