The present study was designed to convert a classical Unani pharmacopoeial preparation ‘Sunun Poste Mughilan’ (a tooth powder) into toothpaste form with the same ingredients as contained in powder form. This work was undertaken with the objective of developing the Standard Operating Procedure (SOP) for manufacturing process of toothpaste. The legitimacy of SOP was determined by assessing the quality of product in three different batches. Each batch was assessed three times for spreadability, foam formation and dispersion time in water. The group which was in range with standard limits was selected as standard batch. The procedures adopted for the preparation of standard batch was taken as the SOP.