Herbal medicines are called as natural products of traditional system of medicines such as Unani, Ayurveda and Siddha. In Unani system of medicine, these herbal products are being prepared using different parts of plants such as root, stem, bark, leaves, flowers and seeds etc. Jawarish-e-Qaiser is one such type of polyherbal Unani formulation prepared by using ten single drugs. The physicians of unani system of medicine have considered this drug as one of the important Unani formulation for the ailments of Qulang (Stomach and Bowel disorder) and Qabz-e-Muzmin (Chronic constipation). The present study was aimed to evaluate pharmacopoeial standards, quality control standards and antimicrobial activity of the drug Jawarish-e-Qaiser. The organisms used in the study were eight type bacterial cultures, namely, Escherichia coli (NCIM 2931), Staphylococcus aureus (NCIM 5021), Enterobacter aerogens (NCIM 5139), Bacillus subtilis (NCIM 2197), Pseudomonas aeruginosa (NCIM 2945), Salmonella typhimurium (NCIM 2501), Bacillus cereus (NCIM 2458), Pseudomonas putida (NCIM 2847) and one fungal (yeast) culture Candida albicans (NCIM 3471). The study revealed that the obtained data of quality control parameters were within the permissible limits of WHO and the drug shows potent antimicrobial activity against all the tested organisms at 100mg/ml concentration. The MIC was found to be within the range of 1.562μg/μl to 3.125μg/μl for majority of the organisms tested. Comparatively the drug was found to be least effective against both the tested Pseudomonas spp.