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Chromatographic Finger Print Analysis of Herbal Drug (Andrographis paniculata Nees) by HPTLC Technique

Manoj Kumar Pandey, Lalit Tiwari, Nitin Rai, Rajeev Kr Sharma and Shivani Sharma

Hippocratic Journal of Unani Medicine
Hippocratic Journal of Unani Medicine, July - September 2012, Vol. 7 No. 3, Pages 77-85


The present study was designed to determine the HPTLC profile of the medicinally important plant Andrographis paniculata Nees. The Chloroform: Methanol (85:15) was employed as mobile phase for phyto-constituents. Linear ascending development was carried out in 
 20 cm x 10 cm twin trough glass chamber (Camag, Mutenz, Switzerland) saturated with the mobile phase and the chromatoplate development for two times with the same mobile phase to get good resolution of phytochemical contents. The developed plate was seen under UV light 254 nm and 366 nm. The methanolic extract of whole parts of Andrographis paniculata Nees showed the presence of 12 different types of phyto-constituents with different Rf. values. The developed HPTLC fingerprints will help the herbal drug industry to distinguish the adulterant and standardization of herbal formulations. Such chemo finger printing will act as biochemical markers for this medicinally important plant in the pharma industry and plant systematic studies.

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