In the past few decades there is growing sense of awareness among the developing world about the importance of traditional systems of medicine such as Unani, Ayurveda and Siddha for maintaining health without side effects. Following this emerged research activities like quality standardization of traditional medicines and development of scientific methods for the manufacture of quality medicine are on the rise. In view of this botanical and physico-chemical standardization of Habb-e-Harsinghar – A Unani formulation has been carried out. Present paper deals with the proper authentication, taxonomic identification, organoleptic characters, ingredient identification, physico-chemical values and chromatographic profile of the drug studied so as to set its standards for quality assurance for global marketing.