Pharmacognostical Evaluation and HPTLC Fingerprinting Studies of Millingtonia Hortensis L. f. Leaf
Mageswari S, Pawan Kumar Sagar, Meera Devi Sri P, Murugeswaran R, Rampratap Meena, Shamshul Arfin and Asiya Khanum
Hippocratic Journal of Unani Medicine
Hippocratic Journal of Unani Medicine April – June 2017, Vol. 12 No. 2, Pages 21-36
Millingtonia hortensis L.f. is commonly known as Cork tree belongs to the family Bignoniaceae. It is an important medicinal plant in Southern Asia such as India, Burma, Thailand and South China. The leaves of the plant were collected freshly and subjected to macro-microscopic, physico-chemical and quality control parameters to fix the authenticity and quality standards of the plant. Microscopical studies showed the presence of wavy epidermal cells with anomocytic stomata; palisade and spongy parenchyma cells; wide pitted vessels with tails at one or both the ends; fibres; glandular trichomes up to 50μ with 16 head cells and unicellular trichomes up to 200μ length. The physico-chemical data showed moisture content as 8.42%, total ash 8.93%, acid insoluble ash 0.098% and alcohol and water soluble extractive values as 5.92% and 24.44% respectively. TLC/HPTLC studies of chloroform and alcohol extracts showed various spots / peaks at 254nm, 366nm and in derivatized plates (Vanillin- sulphuric acid reagent). Quality control parameters such as microbial content and the heavy metals (As, Cd, Pb and Hg) were found to be within the permissible limit. The aflatoxins B1, B2, G1 and G2 were not detected. The study will be useful for the identification and authentication of the plant in dry form as well as in fresh form. The evaluated phytochemical data will serve as pharmacopoeial
standards in the near future for any analytical and biological studies.