Effect of a Combination of Piper cubeba L.f and Cyperus rotundus L. in Experimentally Induced Urolithiasis in Rats

Humaira Bano, *Nasreen Jahan, B.N. Kumar and Saiyed Amrin

Hippocratic Journal of Unani Medicine

Hippocratic Journal of Unani Medicine October - December 2014, Vol. 9 No. 4, Pages 13-26


The present study was undertaken to investigate the effect of a combination of Kabab Chini (Piper cubeba) and Sad Kufi (Cyperus rotundus) in urolithiatic rats. Thirty male Sprague Dawley rats divided into 5 groups of 6 animals each were used. Group I served as control and received regular rat food and drinking water ad libitum. The animals of group II to V were treated with Ethylene glycol (EG) 0.75% (V/V) and ammonium chloride (AC) 1% (W/V) by adding in their drinking water for 7 days to induce urolithiasis. The animals of group II were sacrificed just after 7 days of administration of EG and AC and served as Positive control A, while group III was left untreated till 14 days, and served as Positive control B. The animals of group IV were treated with Cystone (750 mg/kg) and served as Standard control, while the animals of group V were treated with hydro- alcoholic extract of the combination of Piper cubeba and Cyperus rotundus (135mg/ kg) and served as Test group. The treatment continued further for next14 days. At the end of treatment all the animals were sacrificed after 24 hrs of fasting. Highly significant calcium oxalate stones were found in renal tissue as well as in the urine along with increase in urine and serum Calcium, Chloride, Sodium, Phosphorus and creatinine. Test drug significantly decreased the quantity of calcium oxalate deposited in the kidneys and also reverted all the biochemical changes induced by calcium oxalate. The effect of the test drug was found to be comparable to the standard drug Cystone. The study demonstrated a significant lithotriptic effect produced by the combination of Piper cubeba and Cyperus rotunda.

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