Development of HPTLC Finger Prints and Pharmacopoeial Standards of Unani Formulation Jawarish-e-Javed

D. Ramasamy*, Rampratap Meena, S. Mageswari, P. Meera Devi Sri, Shamshad Ahmed Khan, Shamsul Arfin, Syed Jameeluddin Ahmed and Syed Shakir Jamil

Hippocratic Journal of Unani Medicine

Hippocratic Journal of Unani Medicine January - March 2013, Vol. 8 No. 1, Pages 117-127


Standardization of herbal formulations is essential in order to assess the quality of drugs for therapeutic value. The World Health Organization (WHO) has given a detail protocol for the standardization of herbal drugs comprising of a single drugs, but very little literature is available for the standardization of poly-herbal drugs. Jawarish-e-Javed is one of the ancient

most commonly used Unani formulation prescribed for the ailment of stomachic, digestive and brain disorders. The poly herbal drug Jawarish-e-Javed was prepared in different batches with the combination of nine ingredients. Due to lack of scientific standards of the drug the three different batch samples were subjected to evaluate physico-chemical, TLC/HPTLC finger printing, heavy metal, microbial load, aflatoxins and pesticidial residues. The physico-chemical data such as moisture content was 22.46%, alcohol soluble extractives 38.78% and water soluble extractive 38.78% shows presence of polar compounds and inorganic materials respectively. The content of total ash was 1.73% and acid insoluble ash 0.75% shows negligible amount of siliceous matter present in the drug. HPTLC finger prints of chloroform and alcohol extracts shows 13 peaks with the developing systems toluene: ethyl acetate – 9:1 and 6:4 respectively. All three different batch samples were found to be safe when tested for the heavy metal contamination, microbial load, aflatoxins and pesticide residues. The data evolved can be adopted for laying down the pharmacopoeial standards and TLC/ HPTLC finger prints for Jawarish-e-Javed.

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