Authentication of Natural Products by DNA Based Techniques: A Review

Amandeep, K. Bhatia, Ashish K. Kushwaha, Manoj Kumar Panday, Nitin Rai, Lalit Tiwari, and Rajeev Kr. Sharma

Hippocratic Journal of Unani Medicine

Hippocratic Journal of Unani Medicine, July - September 2013, Vol. 8 No. 3, Pages 183-200

Abstract

Herbal drug technology is used for converting botanical materials into medicines, where standardization and quality control with proper integration of modern scientifi c techniques and traditional knowledge is important. The use of chromatographic techniques and marker compounds to standardize botanical preparations has limitations because of their variable sources and chemical complexity. Correct identifi cation and quality assurance is indispensable to ensure reproducible medicinal quality of herbal drugs. Authentication is especially useful in case of those medicinal herbs that are frequently substituted or adulterated with other species or varieties morphologically and phytochemically indistinguishable. DNAbased molecular markers have utility in the fi elds like taxonomy, physiology, embryology, genetics, etc. Extensive research on DNA-based molecular markers is in progress in many research institutes all over the world. DNA -based molecular methods have a great utility in the herbal drug analysis and widely used for the authentication of plant species of medicinal importance. This review provides a brief account of various DNA-based technologies that are useful in genotyping and quick identifi cation of botanicals with suitable examples

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