Antimicrobial Activity of Darchini (Cinnamomum zeylanicum) and Sheesham (Dalbergia sissoo) on Some Common Human Pathogens
Aziz ur Rahman, Tariq Rahim Malik and Tajuddin
Hippocratic Journal of Unani Medicine
Hippocratic Journal of Unani Medicine, July - September 2016, Vol. 11 No. 3, Pages 69-82
The alcoholic and aqueous extracts of cinnamon bark and sissoo wood were evaluated for antibacterial and antifungal activity by disc-agar diffusion technique at three concentration levels (50, 25 and 12.5μg/disc). For evaluation of antibacterial activity different gram positive and gram negative bacterial strains were used viz. Staphylococcus aureus, Streptococcus pyogenes E. coli, Klebsiella sp. Pseudomonas aerugenosa, Proteus sp. Bacillus sp., Shigella sp. and Salmonella typhimurium and to assess antifungal activity the species used are namely Aspergillus flavus, Aspergillus fumigates, Aspergillus niger, Candida albicans, Candida keyfr, Candida kruisei, Candida parapsilosis and Candida tropicalis. The reference drugs used for comparison are Ciprofloxacin (5 μg/disc) for antibacterial potential and Fluconazole (10 μg/disc), Nystatin (100 μg/disc), Amphotericin (100 μg/disc) and Clotrimazole (10 μg/disc) for antifungal activity. Zone of Inhibition was taken as the parameter for the assessment of antimicrobial activity. The alcoholic extract of Cinnamomum zeylanicum showed significant antibacterial effect against Streptococcus pyogenes E. coli and Pseudomonas aerugenosa while the alcoholic and aqueous extracts of Dalbergia sissoo exhibited significant effect against all the tested bacterial strains at the different doses. The extract of both the test drugs also gives significant effect against different fungal strains used. Furthermore, to confirm the originality and quality of the test drugs the samples were also screened on the basis of certain physico-chemical parameters.